Matrigel plug assay: evaluation of the angiogenic response by reverse transcription-quantitative PCR
نویسندگان
چکیده
منابع مشابه
The deficits of matrigel plug assay
| October 2014 | 1018 cells in the matrigel plug (local administration) is not suitable for statically. If the leptin levels were measured before and after matrigel administration, it should have been more meaningful. Because 10-day application of matrigel plug with leptin can’t affect the systemic leptin results. In addition, as shown in recent studies, high fat diet-induced obesity causes inc...
متن کاملMagnetic quantitative reverse transcription PCR: a high-throughput method for mRNA extraction and quantitative reverse transcription PCR.
Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable. One method that stands out with respect to free up- or downscaling of sample size and reliability is the isolation of mRNA using oligodeoxythymidylate [oligo(dT)25]-coated magnetic particles. In comb...
متن کاملDevelopment and assessment of a quantitative reverse transcription-PCR assay for simultaneous measurement of four amplicons.
BACKGROUND High-throughput and forward-deployable biological dosimetry capabilities are required for tactical and medical decisions after radiologic events. We previously reported a quantitative reverse transcription (QRT)-PCR assay for human radiation-responsive gene targets using a whole-blood ex vivo irradiation model, but we needed a multitarget assay on a smaller, less costly, real-time PC...
متن کاملDevelopment of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.
A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.
متن کاملSensitive detection of multiple rotavirus genotypes with a single reverse transcription-real-time quantitative PCR assay.
Rotaviruses are one of the major causes of diarrhea in infants and children under 5 years old, especially affecting developing countries. In natural disasters, fecal matter and potable waters can mix, allowing low, yet infective, concentrations of rotavirus to be present in water supplies, constituting a risk for the population. Any of the most commonly detected rotavirus genotypes could origin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Angiogenesis
سال: 2012
ISSN: 0969-6970,1573-7209
DOI: 10.1007/s10456-012-9324-7